The presenter, 50, was talking about one of the contestants playing the violin when the rude hand movement slipped out.
The Britain’s Got Talent judge unintentionally made the gesture while the panel were discussing if that week’s performer – known as Songbird – could really sing.
Oops! Amanda Holden accidentally made an X-rated gesture on I Can See Your Voice while talking about playing the violin on Saturday
The celebrities were attempting to help the contestants Sophie and Josh solve the mystery by deducing if the singer had any musical talent.
‘You said she’s a multi instrumentalist?’ Jimmy Carr questioned the show’s host Paddy McGuinness who said he was correct.
‘I imagine, if that’s true, she can hold a tune?’ Jimmy deduced from the little evidence he had.
Cracking up: The presenter left Ricky Wilson and Alison Hammond crying with laughter when the rude hand movement slipped out
But the Kaiser Chiefs frontman Ricky was suspicious of the comedians logic, saying: ‘They didn’t have a close up on the fingers though.’
Amanda then weighed in and attempted to demonstrate how the musician had held the instrument but looked like she was doing something far more risque.
As the Britain’s Got Talent judge moved her fingers around Jimmy cackled and yelled: ‘It’s a family show!’
Embarrassed Amanda quickly scrambled to explain herself amongst the giggles and exclaimed: ‘No! You know what I mean though?…When your fingers shake on the violin?!’
‘We all know what you mean,’ replied Paddy coyly. ‘But let’s get back to the singing.’
X-rated: Amanda’s co-star Jimmy Carr reminded her the programme was a ‘family show’ after her explicit finger action
The saucy moment comes after it was revealed earlier this month that Amanda is set to star in her own series called No Holden Back on E4.
The presenter, who previously teased she was working on a secret project, said she is ‘delighted’ to have landed her own show.
The eight-part series will follow Amanda’s ‘unique relationship’ with her Nan Myrtle, with the star moving back in with her beloved grandmother.
Amanda told The Sun: ‘I’m delighted that everyone is finally going to meet my nan, the apple never falls far from my tree and in this series she will certainly be keeping me on my toes.’
Show: The saucy moment comes after it was revealed earlier this month that Amanda is set to star in her own series called No Holden Back on E4
Embrace sunny hues like Amanda wearing L.K.Bennett
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Once again, the latter turned to the trend by dressing up in L.K.Bennett’s ‘Amalia’ dress for another day of presenting Heart radio in style thanks to Karl Willett.
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A spokesperson for her nan, Myrtle, said: ‘I have no comment, apart from I’m really hoping being on TV might mean I can finally meet my favourite young man, Ben Shephard.’
Channel 4 Commissioning Editor, Steven Handley told the publication: ‘Amanda is one of the UK’s most well-known stars and we are thrilled to get insight into her life with her nan.’
MailOnline has contacted Amanda’s representatives and Channel 4 for further comment.
The show will also be broadcast on Channel 4 meaning Amanda will have programmes on ITV (Britain’s Got Talent), BBC (I Can See Your Voice) and Channel 4 (No Holden Back).
Reality series: The presenter said she is ‘delighted’ to have landed her own show No Holden Back (pictured with her daughters Alexa, 15, and Hollie, nine, in March
Source: Daily Mail